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Effect of Tropaeolum Tuberosum "Mashua" (Tropaeolaceae) on gene expression related to spermatogenesis in mouse

Efecto de Tropaeolum Tuberosum "Mashua" (Tropaeolaceae) sobre la expresión génica relacionada a Espermatogénesis en ratón

Authors

  • José Gonzales Daga Laboratory of Reproduction and Developmental Biology. Instituto de Ciencias Biológicas “Antonio Raimondi” (ICBAR). Universidad Nacional Mayor de San Marcos (UNMSM). Lima, Peru.
  • José Pino Gaviño Laboratory of Reproduction and Developmental Biology. Instituto de Ciencias Biológicas “Antonio Raimondi” (ICBAR). Universidad Nacional Mayor de San Marcos (UNMSM). Lima, Peru; Centro de Investigación de Recursos Naturales (CIRNA). Vice-Rectorate of Research and Postgraduate Studies. UNMSM.
  • Rafael Alvis Dávila Centro de Investigación de Recursos Naturales (CIRNA). Vice-Rectorate of Research and Postgraduate Studies. UNMSM.
  • Juan Francia Quiroz Universidad Científica del Sur. Lima, Peru.
  • Carlos Bell Cortez Laboratory of Analytical Chemistry. Faculty of Pharmacy and Biochemistry. UNMSM. Lima, Peru.
  • Pilar Pino Velásquez Laboratory of Reproduction and Developmental Biology. Instituto de Ciencias Biológicas “Antonio Raimondi” (ICBAR). Universidad Nacional Mayor de San Marcos (UNMSM). Lima, Peru.
  • Betty Shiga Oshige Laboratory of Reproduction and Developmental Biology. Instituto de Ciencias Biológicas “Antonio Raimondi” (ICBAR). Universidad Nacional Mayor de San Marcos (UNMSM). Lima, Peru; Centro de Investigación de Recursos Naturales (CIRNA). Vice-Rectorate of Research and Postgraduate Studies. UNMSM. 3Universidad Científica del Sur. Lima, Peru.

DOI:

https://doi.org/10.25176/RFMH.v23i3.5907

Keywords:

spermatogenesis, genic expression, mashua, mouse, Tropaeolum tuberosum, medicinal plants.

Abstract

Introduction: Tropaeolum tuberosum, known as "mashua" is an Andean tuber that holds both economic and nutritional value for low-income populations. It is believed that it affects male fertility because Andean men associate it with impotence and decreased fertility. Studies conducted on rats fed with "mashua" showed that there was a 45% decrease in the testosterone/dihydrotestosterone ratio. The effect of this plant on reproduction is related to its content of isothiocyanates, compounds that covalently bind to proteins, which may be directly or indirectly involved in the spermatogenic process. The purpose of this research was to evaluate the effect of the aqueous extract of "mashua" on spermatogenesis and reproductive physiology of mice. Methodology: In vivo morphofunctional parameters of mouse sperm (spermatogram) were evaluated and the expression of Cytochrome P450 17α-hydroxylase/17,20-lyase, acute steroidogenesis regulatory protein, cyclin, and protamine related to spermatogenesis was quantified.

Results: The results indicated that at 7, 14 and 21 days of dosing, the sperm count was affected, as well as their progressive motility (PM), on the other hand, a delay in their maturation was observed. Regarding gene expression, no significant differences were found between the expression of the two genes studied (Cytochrome P450 17α-hydroxylase/17,20-lyase, Cyclin), Conclusion: The effect of "mashua" does not occur at the level of gene expression involved in spermatogenesis, but at the level of its functions as a protein.

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Author Biographies

José Gonzales Daga , Laboratory of Reproduction and Developmental Biology. Instituto de Ciencias Biológicas “Antonio Raimondi” (ICBAR). Universidad Nacional Mayor de San Marcos (UNMSM). Lima, Peru.

Master in Molecular Biology

José Pino Gaviño , Laboratory of Reproduction and Developmental Biology. Instituto de Ciencias Biológicas “Antonio Raimondi” (ICBAR). Universidad Nacional Mayor de San Marcos (UNMSM). Lima, Peru; Centro de Investigación de Recursos Naturales (CIRNA). Vice-Rectorate of Research and Postgraduate Studies. UNMSM.

Biologist

Rafael Alvis Dávila, Centro de Investigación de Recursos Naturales (CIRNA). Vice-Rectorate of Research and Postgraduate Studies. UNMSM.

Biologist

Juan Francia Quiroz , Universidad Científica del Sur. Lima, Peru.

Biologist

Carlos Bell Cortez, Laboratory of Analytical Chemistry. Faculty of Pharmacy and Biochemistry. UNMSM. Lima, Peru.

Doctor in Pharmacy and Biochemistry

Pilar Pino Velásquez, Laboratory of Reproduction and Developmental Biology. Instituto de Ciencias Biológicas “Antonio Raimondi” (ICBAR). Universidad Nacional Mayor de San Marcos (UNMSM). Lima, Peru.

Master in Assisted Reproduction Procedures

Betty Shiga Oshige, Laboratory of Reproduction and Developmental Biology. Instituto de Ciencias Biológicas “Antonio Raimondi” (ICBAR). Universidad Nacional Mayor de San Marcos (UNMSM). Lima, Peru; Centro de Investigación de Recursos Naturales (CIRNA). Vice-Rectorate of Research and Postgraduate Studies. UNMSM. 3Universidad Científica del Sur. Lima, Peru.

Biologist

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How to Cite

Gonzales Daga , J. ., Pino Gaviño , J. ., Alvis Dávila, R. ., Francia Quiroz , J. ., Bell Cortez, C. ., Pino Velásquez, P. ., & Shiga Oshige, B. . (2023). Effect of Tropaeolum Tuberosum "Mashua" (Tropaeolaceae) on gene expression related to spermatogenesis in mouse: Efecto de Tropaeolum Tuberosum "Mashua" (Tropaeolaceae) sobre la expresión génica relacionada a Espermatogénesis en ratón. Revista De La Facultad De Medicina Humana, 23(3), 68–72. https://doi.org/10.25176/RFMH.v23i3.5907 (Original work published September 14, 2023)