OBTAINING VIRUS FREE PLANTS FROM ASPARAGUS OFFICINALIS L. “Asparagus” IN VITRO

Authors

  • Mauro Quiñonez Aguilar Universidad Ricardo Palma
  • Renzo Riboty Ilas Universidad Ricardo Palma

DOI:

https://doi.org/10.31381/scientia.v21i21.2763

Keywords:

Asparagus officinalis L. / micropropagation / 6-Benzyl Aminopurine/ Meristem

Abstract

This work attempts to obtain free virus Asparagus officinalis L. “garden asparagus”
plants, applying the biotechnological methods of the in vitro meristem culture. Axillary
buds were used as explants, and were sterilized, isolated, cultivated in the basal
medium Murashige & Skoog (MS) at 3 different concentrations (0,5; 1,0 & 1,5 mg/L)
of the phytohormone 6-Bencil Aminopurine (6-BAP), and incubated under controlled
conditions in a cultivation room. As a result, 8 shoots & 8 cladodes, 6 shoots with no
cladodes and 1 shoot & 5 cladodes were obtained in the culture mediums supplemented
with the 3 concentrations of 6-BAP respectively.

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Author Biography

Mauro Quiñonez Aguilar, Universidad Ricardo Palma

Ingeniero Agroquímico Edafólogo (Agrónomo). Ph.D. en Ciencias Biológicas (Bioquímica). Estudios de Especialización en Biotecnología e Ingeniería Genética de plantas - Universidad Estatal de Agricultura Timiryazev, Moscú, Rusia. Agricultural Biotechnolgy Short Course: Bioseguridad, Propiedad Intelectual y Patentes, Regulación de los cultivos transgénicos Michigan State University, USA, EE:UU. Cochran Fellowship Program. Has Completed Training in Biotechnology - United States Department of Agriculture EE.UU. International Short Curse in Biofuels - Michigan State University and Great Lakes Bioenergy Research Center. Ha sido Director de la Oficina Biotecnología del CONCYTEC. Director General de OFOPCYT del CONCYTEC. Representante del Perú ante la UNESCO y REDBIO. Coordinador de CONCYTEC ante ICGEB. Representante de CONCYTEC - RELAB, Asesor del Jefe de INIA y Jefe del Laboratorio de Biotecnología de CICH de INIA Huaral.
Director técnico y fundador de COBIASA. Fundador del Laboratorio de Biotecnología de la URP. Evaluador externo de proyectos de investigación de FINCyT, FONDECYT, CONCYTEC, PINEA, entre otras instituciones nacionales e internacionales.

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Published

2020-01-27

How to Cite

Quiñonez Aguilar, M., & Riboty Ilas, R. (2020). OBTAINING VIRUS FREE PLANTS FROM ASPARAGUS OFFICINALIS L. “Asparagus” IN VITRO. Scientia, 21(21), 119–126. https://doi.org/10.31381/scientia.v21i21.2763

Issue

Vol. 21 No. 21 (2019): SCIENTIA

Section

Ciencias