PHENOTYPIC AND GENOTIPIC DETECTION OF ESCHERICHIA COLI PRODUCER OF β-LACTAMASES EXTENDED SPECTRUM ISOLATED FROM BIRDS SUPPLY IN PERU

Abstract

Bacterial resistance to antibiotics has become an important public health issue that involves human medicine, veterinary medicine, food and environmental safety in Perú. This work, developed in a private laboratory located in the city of  Lima - Peru,  focused on the phenotypic and genotypic detection of Escherichia coli (Escherich, 1885) strains that produce extended spectrum β-lactamase enzymes (βLEE), isolated in poultry. A total of 185 isolated bird samples of E. coli from 26 intensive breeding companies located in Peru were evaluated. The results reflected that 38.4% of strains with the presence of ESBL confirmed phenotypically; Likewise, genes were expressed from total DNA by polymerase chain reaction (PCR) obtaining 33.5% between the blaTEM, blaSHV and blaCTX-M-1 genes and 4.9% of E. coli strains producing βLEE phenotypic, no gene bla determined. As a conclusion, there is a high presence of E. coli strains that produce extended-spectrum β-lactamase enzymes (βLEE), with the blaCTX-M-1 gene being the most frequent.