Kinetics of the release of platelet-derived growth factor (PDGF-BB) derived from human umbilical cord platelet-rich plasma.: Cinética de la liberación del factor de crecimiento derivado de plaquetas (PDGF-BB) derivado del plasma rico en plaquetas de cordón umbilical humano  |   人类脐带血小板富集血浆中血小板衍生生长因子（PDGF-BB）释放的动力学

Pedro Aro Guardia; Ana  Peralta Antialón; Andrea  Rodríguez Chávez; Christian  Lezama Martinez; Carmen Palomino Ccorimanya; William  Bocangel Espinoza; Rodrigo  Paredes de la Fuente; José Aguilar Olano

doi:10.25176/RFMH.v24i3.6703

Authors

Pedro Aro Guardia Servicio de Hemoterapia y Banco de Sangre del Hospital Nacional Cayetano Heredia Universidad Peruana Cayetano Heredia Internal Medicine Unit, Mount Sinai Beth Israel Hospital
Ana Peralta Antialón Universidad Peruana Cayetano Heredia
Andrea Rodríguez Chávez Universidad Peruana Cayetano Heredia
Christian Lezama Martinez Universidad Peruana Cayetano Heredia
Carmen Palomino Ccorimanya Universidad Peruana Cayetano Heredia
William Bocangel Espinoza Universidad Científica del Sur
Rodrigo Paredes de la Fuente Unidad de Medicina Interna, Hospital Mount Sinai Beth Israel
José Aguilar Olano Laboratorios de Inmunología de la Universidad Peruana Cayetano Heredia

DOI:

https://doi.org/10.25176/RFMH.v24i3.6703

Keywords:

Platelets-rich plasma, platelet-derived growth factor, umbilical cord, blood

Abstract

Introduction: The use of cord blood (UC) offers numerous advantages in regenerative medicine. Platelet-rich plasma (PRP) is a source of growth factors where its dynamics over time have been evaluated in peripheral blood; however, its evaluation in UC blood has not been studied. Objective: To evaluate the kinetics of PDGF-BB release from PRP obtained from UC blood. Materials and methods: In vitro experimental study. UC blood samples were collected from 6 term deliveries of healthy pregnant women between 18 and 36 years of age who attended the Cayetano Heredia Hospital in Lima-Peru. The samples obtained were centrifuged at 900 g for 10 minutes to prepare the PRP. Activation was performed with 10% calcium gluconate (GLu.Ca) and divided into 4 aliquots: (i) without activator; (ii) 1 hour; (iii) 24 hours; (iv) 48 hours. PDGF-BB quantification was evaluated by ELISA method. Results: The mean ± standard deviation of PDGF-BB concentration measured at 1 hour, 24 hours and 48 hours were 6127.9 ± 101.6, 6197.5 ± 34 and 6176.8 ± 63.3 respectively. The kinetics of PDGF-BB released by PRP showed constant values during the 48 hours. Conclusions: PDGF-BB release from PRP obtained from UC blood was rapidly induced and remained constant and sustained during the first 48 hours after activation.

Downloads

Download data is not yet available.

Author Biographies

Pedro Aro Guardia, Servicio de Hemoterapia y Banco de Sangre del Hospital Nacional Cayetano Heredia Universidad Peruana Cayetano Heredia Internal Medicine Unit, Mount Sinai Beth Israel Hospital

Médico Cirujano

Ana Peralta Antialón, Universidad Peruana Cayetano Heredia

Químico Farmacéutico

Andrea Rodríguez Chávez, Universidad Peruana Cayetano Heredia

Químico Farmacéutico

Christian Lezama Martinez, Universidad Peruana Cayetano Heredia

Tecnólogo Médico

Carmen Palomino Ccorimanya, Universidad Peruana Cayetano Heredia

Tecnólogo Médico

William Bocangel Espinoza, Universidad Científica del Sur

Estudiante

Rodrigo Paredes de la Fuente, Unidad de Medicina Interna, Hospital Mount Sinai Beth Israel

Médico Cirujano

José Aguilar Olano, Laboratorios de Inmunología de la Universidad Peruana Cayetano Heredia

Médico Reumatólogo

References

Hashemi SS, Mahmoodi M, Rafati AR, Manafi F, Mehrabani D. The Role of Human Adult Peripheral and Umbilical Cord Blood Platelet-Rich Plasma on Proliferation and Migration of Human Skin Fibroblasts. World J Plast Surg. 2017 May;6(2):198-205.

Roh YH, Kim W, Park KU, Oh JH. Cytokine-release kinetics of platelet-rich plasma according to various activation protocols. Bone Joint Res. 2016;5(2):37-45

Murphy MB, Blashki D, Buchanan RM, Yazdi IK, Ferrari M, Simmons PJ, et al. Adult and umbilical cord blood-derived platelet-rich plasma for mesenchymal stem cell proliferation, chemotaxis, and cryo-preservation. Biomaterials. 2012; 33(21): 5308–5316.

Casati L, Celotti F, Negri-Cesi P, Sacchi MC, Castano P, Colciago A. Platelet derived growth factor (PDGF) contained in Platelet Rich Plasma (PRP) stimulates migration of osteoblasts by reorganizing actin cytoskeleton. Cell AdhMigr. 2014;8(6):595-602.

Wu PK, Diaz R, Borg-Stein J. Platelet-Rich Plasma. Physical Medicine and Rehabilitation Clinics of North America. 2016;27(4):825-53.

Charousset C, Zaoui A, Bellaiche L, Bouyer B. Are multiple platelet-rich plasma injections useful for treatment of chronic patellar tendinopathy in athletes? a prospective study. Am J Sports Med. 2014;42:906-911.

Dhurat R, Sukesh M. Principles and methods of preparation of platelet-rich plasma: a review and author’s perspective. J Cutan Aesthet Surg. 2014;7(4):189–97.

Khurshid Z, Asiri FY, Najeeb S, Ratnayake J. The Impact of Autologous Platelet Concentrates on the Periapical Tissues and Root Development of Replanted Teeth: A Systematic Review. Materials. 2022; 15(8):2776

Ramaswamy Reddy SH, Reddy R, Babu NC, Ashok GN. Stem-cell therapy and platelet-rich plasma in regenerative medicines: A review on pros and cons of the technologies. J Oral Maxillofac Pathol. 2018;22(3):367-374

Lyras DN, Kazakos K, Agrogiannis G, Verettas D, Kokka A, Kiziridis G, et al. Experimental study of tendon healing early phase: Is IGF-1 expression influenced by platelet rich plasma gel? Orthop Traumatol Surg Res. 2010;96:381–7.

Shi GJ, Shi GR, Zhou JY, Zhang WJ, Gao CY, Jiang YP, et al. Involvement of growth factors in diabetes mellitus and its complications: A general review. Biomed Pharmacother. 2018;101:510-527.

Romanov YA, Vtorushina VV, Dugina TN, Romanov AY and Petrova NV. Human Umbilical Cord Blood Serum/Plasma: Cytokine Profile and Prospective Application in Regenerative Medicine. Cell Technologies in Biology and Medicine. 2019;3:173-177

Buzzi M, Versura P, Grigolo B, Cavallo C, Terzi A, Pellegrini M, et al. Comparison of growth factor and interleukin content of adult peripheral blood and cord blood serum eye drops for cornea and ocular surface diseases. Transfus Apher Sci. 2018;57:549–55.

Parazzi V, Lavazza C, Boldrin V, Montelatici E, Pallotti F, Marconi M, et al. Extensive Characterization of Platelet Gel Releasate From Cord Blood in Regenerative Medicine. Cell Transplant. 2015;24(12):2573–84.

Mazzocca A, McCarthy M, Chowaniec D. Platelet-rich plasma differs according to preparation method and human variability. J Bone Joint Surg. 2012;94-A:308-316.

Lee JW, Kwon OH, Kim TK, Cho YK, Choi KY, Chung HY, et al. Platelet-rich plasma: quantitative assessment of growth factor levels and comparative analysis of activated and inactivated groups. Arch Plast Surg. 2013;40(5):530-5.

Van den Dolder J, Mooren R, Vloon AP, Stoelinga PJ, Jansen JA. Platelet-rich plasma: quantification of growth factor levels and the effect on growth and differentiation of rat bone marrow cells. Tissue Eng. 2006;12(11):3067-73.

Eppley BL, Woodell JE, Higgins J. Platelet Quantification and Growth Factor Analysis from Platelet-Rich Plasma: Implications for Wound Healing. Plastic and Reconstructive Surgery. 2004; 114(6):1502-1508

Josh F, Tobita M, Tanaka R, Orbay H, Ogata K, Suzuki K, et al. Concentration of PDGF-AB, BB and TGF-β1 as valuable human serum parameters in adipose-derived stem cell proliferation. J Nippon Med Sch. 2013;80:140–147.

Stojek M, Adrych K, Rojek L, Smoczynski M, Sledzinski T, Szrok S, et al. Decreased serum platelet derived growth factor BB levels in acute and increased in chronic pancreatitis. World J Gastroenterol. 2014;20(36):13127-32.

Munawirah A, Esa T, Bahrun U. Analysis of Platelet Counts and Platelet-Derived Growth Factor-Bb Levels in Platelet Rich Plasma Produced with EDTA as Anticoagulant in Three Different Centrifugation Methods. Indian Journal of Public Health Research & Development. 2020; 11(6):1204-1209

Amable PR, Carias RB, Teixeira MV, da Cruz Pacheco I, Corrêa do Amaral RJ, Granjeiro JM, et al. Platelet-rich plasma preparation for regenerative medicine: optimization and quantification of cytokines and growth factors. Stem Cell Res Ther. 2013;4(3):67

Yan L, Zhou L, Yan B, Zhang L, Du W, Liu F, et al. Growth factors-based beneficial effects of platelet lysate on umbilical cord-derived stem cells and their synergistic use in osteoarthritis treatment. Cell Death Dis..2020; 11: 857

Steller D, Herbst N, Pries R, Juhl D, Hakim SG. Impact of incubation method on the release of growth factors in non-Ca2+-activated PRP, Ca2+-activatedPRP, PRF and A-PRF. J Craniomaxillofac Surg. 2019;47(2):365-72.

Verma R, Kandwal A, Negi G, Chandra H. Factors affecting the quantity and quality of platelet-rich plasma and platelet-derived growth factor-BB: an observational study. Journal of Bio-X Research. 2021;4(2):67-70

Mariani E, Pulsatelli L, Cattini L, Dolzani P, Assirelli E, Cenacchi A, et al. Pure Platelet and Leukocyte–Platelet-Rich Plasma for Regenerative Medicine in Orthopedics—Time- and Preparation-Dependent Release of Growth Factors and Effects on Synovial Fibroblasts: A Comparative Analysis. International Journal of Molecular Sciences. 2023; 24(2):1512.

Roffi A, Filardo G, Assirelli E, Cavallo C, Cenacchi A, Facchini A, et al. Does Platelet-Rich Plasma Freeze-Thawing influence growth factor release and their effects on chondrocytes and synoviocytes?. BioMed Research International. 2014; 2014: 692913.

Collins T, Alexander D and Barkatali B. Platelets-Rich plasma: a narrative review. Effort Open Reviews. 2021; 6(4): 225-235.

Da Silva L, Huber SC, Montalvão SA, Bassora FD, de Paula EV, Annichino-Bizzacchi J. Platelet Activation Is Not Crucial for Platelet-Rich Plasma (PRP), When Used As Autologous Therapeutic Product, and Could be Lyophilized without Any Growth Factor Loss. Blood. 2016;128(22):2639–9 Kazuhisa Nakashima yAkira Nifuji

Komatsu K, Ideno H, Shibata T, Nakashima K, Nifuji A. Platelet-derived growth factor-BB regenerates functional periodontal ligament in the tooth replantation. Sci Rep. 2022;12:3223-3239

Evrova O. and Buschmann J. In vitro and in vivo effects of PDGF-BB delivery strategies on tendon healing: a review. European Cells and Materials.2017;34:15-39.

Calleja-Blanco S, Abaroa C, Molina C, Trincado M. Detection and Quantification of Platelet-Derived Growth Factor in Circulating Blood Concentrates vs Platelet-Rich Fibrin. Journal of oral and Maxillofacial Surgery. 2020:78(10):E96

Borena BM, Martens A, Broeckx SY, Meyer E, Chiers K, Duchateau L, et al. Regenerative skin wound healing in mammals: state-of-the-art on growth factor and stem cell-based treatments. Cell Physiol Biochem. 2015; 36:1-23

Lee K, Silva EA, Mooney DJ. Growth factor delivery-based tissue engineering: general approaches and a review of recent developments. J R Soc Interface. 2011;8:153-170

Schär MO, Diaz-Romero J, Kohl S, Zumstein MA, Nesic D. Platelet-rich concentrates differentially release growth factors and induce cell migration in vitro. Clin Orthop Relat Res. 2015;473(5):1635-43