Kinetics of the release of platelet-derived growth factor (PDGF-BB) derived from human umbilical cord platelet-rich plasma.: Cinética de la liberación del factor de crecimiento derivado de plaquetas (PDGF-BB) derivado del plasma rico en plaquetas de cordón umbilical humano

Pedro Alberto Aro Guardia; Ana  Peralta; Andrea  Nisi; Christian  Lezama; Carmen Palomino; William  Bocangel; Rodrigo  Paredes; José Aguilar

doi:10.25176/RFMH.v24i3.6703

Autores/as

Pedro Alberto Aro Guardia Hemotherapy Service and Blood Bank, National Hospital Cayetano Heredia Cayetano Heredia University Internal Medicine Unit, Mount Sinai Beth Israel Hospital
Ana Peralta Cayetano Heredia University
Andrea Nisi Universidad Peruana Cayetano Heredia
Christian Lezama Universidad Peruana Cayetano Heredia
Carmen Palomino Universidad Peruana Cayetano Heredia
William Bocangel Scientific University of the South
Rodrigo Paredes Internal Medicine Unit, Mount Sinai Beth Israel Hospital
José Aguilar Immunology Laboratories, Cayetano Heredia University

DOI:

https://doi.org/10.25176/RFMH.v24i3.6703

Palabras clave:

Plasma rico en plaquetas, Factor de crecimiento derivado de plaquetas, cordon umbilical, sangre

Resumen

Introducción: El uso de sangre de cordón umbilical (CU) tiene muchas ventajas en la medicina regenerativa. El plasma rico en plaquetas (PRP) es una fuente de factores de crecimiento en donde su dinámica en el tiempo ha sido evaluada en sangre periférica, sin embargo, su evaluación en sangre de CU no ha sido estudiada. Objetivo: Evaluar la cinética de la liberación del PDGF-BB del PRP obtenido de sangre de CU. Materiales y métodos: estudio experimental in vitro. Se recolectaron muestras de sangre de CU de 6 partos a término de gestantes que tenían entre 18 y 36 años sanos que acudieron al Hospital Cayetano Heredia en Lima-Perú. Las muestras obtenidas se centrifugaron a 900 g durante 10 minutos para preparar el PRP. Su activación se realizó con gluconato de calcio (GLu.Ca) al 10% y se dividió en 4 alícuotas: (i) sin activador. (ii) 1 hora. (iii) 24 horas. (iv) 48 horas. La cuantificación del PDGF-BB fue evaluada mediante el método de ELISA. Resultados: La media ± desviación estándar de la concentración de PDGF-BB medido a la hora, 24 horas y 48 horas fue 6127.9 ± 101.6, 6197.5 ± 34 y 6176.8 ± 63.3 respectivamente. La cinética del PDGF-BB liberado por el PRP mostró valores constantes durante las 48 horas. Conclusiones: La liberación de PDGF-BB del PRP obtenido de sangre de CU se indujo rápidamente y se mantuvo constante y de forma mantenida durante las primeras 48 horas luego de su activación.

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Biografía del autor/a

Pedro Alberto Aro Guardia, Hemotherapy Service and Blood Bank, National Hospital Cayetano Heredia Cayetano Heredia University Internal Medicine Unit, Mount Sinai Beth Israel Hospital

Medical Doctor

Ana Peralta, Cayetano Heredia University

Pharmaceutical Chemist

Andrea Nisi, Universidad Peruana Cayetano Heredia

Pharmaceutical Chemist

Christian Lezama, Universidad Peruana Cayetano Heredia

Medical Technologist

Carmen Palomino, Universidad Peruana Cayetano Heredia

Medical Technologist

William Bocangel, Scientific University of the South

Student

Rodrigo Paredes, Internal Medicine Unit, Mount Sinai Beth Israel Hospital

Medical Doctor

José Aguilar, Immunology Laboratories, Cayetano Heredia University

Rheumatologist

Citas

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