Iron-deficient diet as an experimental model to induce anemia in Balb/c mice
Dieta deficiente en hierro como modelo experimental para inducir anemia en ratones Balb/c
DOI:
https://doi.org/10.25176/RFMH.v2025i1.6657Keywords:
Anemia, iron deficiency, hemoglobins, models, animalsAbstract
In order to develop an experimental model of anemia in Balb/c mice using an iron-deficient diet, an experimental and longitudinal study was conducted with 16 male Balb/c mice, four weeks old, divided into two groups: one with a standard diet and the other with an iron-deficient diet (2.83 mg/Kg). Hemoglobin levels and weight were measured at baseline and after 30 days using an EKF Diagnostic® hemoglobinometer and a Soehnle® digital scale. The Student’s t-test for independent samples was used for statistical analysis, with p<0.05 as the significance level. No significant differences were found initially; however, after 30 days, the iron-deficient diet group showed a significant reduction in
hemoglobin (5.30±1.67 g/dL vs. 15.65±0.96 g/dL; p<0.001) and weight (28.63±6.19 g vs. 38.13±2.167 g; p<0.003). The experimental model successfully induced anemia in Balb/c mice, providing a reproducible tool for preclinical studies.
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